MXIS 2017

Practical course on in-plate crystallography, with special focus on ligand screening

November-December 2017

 

The MXIS training course is dedicated to in situ protein crystallography, with a special emphasis on ligand screening. This workshop is organized in two parts, each one lasting for 3 days. It includes:

 

  • Part 1 (Montpellier): full lectures at the “Centre de Biochimie Structurale” followed by practicals. Topics: plate pre-coating for “Dry” co-crystallization [1], in situ screening on a lab source).
  • Part 2 (Grenoble): full lectures at the “Institut de Biologie Structurale” and practical sessions at the ESRF, on beamlines FIP-BM30A and ID30B. Full classes will be done in day 1 while practical sessions will be organized in subgroups, from day 2 to day 3. Topics: in situ diffraction for crystal screening and structure resolution, in situ data processing.

Possibility will be given to participants to practice on their own samples. A fragment library may be available to be used on your sample (to be confirmed later on)

 

The workshop is limited to 6 and 8 participants for Part 1 and 2 respectively.

No fees are required.

 

The application period will close on the 15th October. Applicants will be required to submit a short CV and a motivation letter to jean-luc.ferrer@ibs.fr

 

More information on https://workshops.ibs.fr/MXIS-2017

 

[1] Combining 'dry' co-crystallization and in situ diffraction to facilitate ligand screening by X-ray crystallography. Gelin M, Delfosse V, Allemand F, Hoh F, Sallaz-Damaz Y, Pirocchi M, Bourguet W, Ferrer JL, Labesse G, Guichou JF. Acta Crystallogr D Biol Crystallogr. 2015 Aug;71(Pt 8):1777-87. doi: 10.1107/S1399004715010342.