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Practicals

The practical session in the afternoon will start with a general presentation of the platforms that will be used in the different practical workshops (for a description of the workshops content, see below).

Participants can only attend one of the workshop and sessions are limited to a maximum of participants (see below).  Selection will be on a first come first serve basis.

AFM (IBS) (max: 5 participants)

Getting familiar with the possibility of imaging single isolated molecules with Atomic Force Microscopes. The practical training will include sample preparation, microscope settings (cantilever mounting), imaging with several different modes, and minimal image analysis. For this session, AFM imaging will be performed in air.

M4D/Super-resolution microscopy (IBS) (max: 5 participants)

We will demonstrate STORM super-resolution fluorescence microscopy based on click chemistry labeling of the bacterial peptidoglycan. The principle of the technique will be explained, and data will be collected and processed.

Study of thin cell sections (IBS) (max: 4 participants)

This practical session will start with some introduction on cellular sample preparation and then we will show you how to generate ultra-thin sections of resin-embedded cells. Finally, you will have access to the electron microscope to have a look at the ultrastructure of the cells (bacteria, cells or tissue will be available).

CryoEM (EMBL) (max: 5 participants)

X-ray/neutron tomographic reconstruction (ESRF/ILL) (max: 10 participants)

The practicals will start with a brief reminder on the basic principles on how to reconstruct a virtual volume image with radiographic projections. A demonstration will follow with real synchrotron data, underlining the specific contrast modes incl. simple phase-retrieval applications. For the final part, a simple tomographic reconstruction tool can be used by the participants in order to get a first insight into the procedures.